ID   CP2DP_PIG               Reviewed;         500 AA.
AC   O46658;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-NOV-2008, entry version 59.
DE   RecName: Full=Vitamin D(3) 25-hydroxylase;
DE            EC=1.14.13.15;
DE   AltName: Full=Cytochrome P450 2D25;
DE   AltName: Full=CYPIID25;
GN   Name=CYP2D25;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC   Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-57; 249-273 AND
RP   408-430.
RC   TISSUE=Liver;
RX   MEDLINE=98086378; PubMed=9425298; DOI=10.1006/bbrc.1997.7551;
RA   Postlind H., Axen E., Bergman T., Wikvall K.;
RT   "Cloning, structure, and expression of a cDNA encoding vitamin D3 25-
RT   hydroxylase.";
RL   Biochem. Biophys. Res. Commun. 241:491-497(1997).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-17.
RC   TISSUE=Liver;
RX   MEDLINE=93075023; PubMed=1445236;
RA   Axen E., Bergman T., Wikvall K.;
RT   "Purification and characterization of a vitamin D3 25-hydroxylase from
RT   pig liver microsomes.";
RL   Biochem. J. 287:725-731(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-11.
RC   TISSUE=Liver;
RX   MEDLINE=91316123; PubMed=1859829; DOI=10.1016/0167-4838(91)90161-R;
RA   Sono H., Sonoda Y., Sato Y.;
RT   "Purification and characterization of cytochrome P-45014DM (lanosterol
RT   14 alpha-demethylase) from pig liver microsomes.";
RL   Biochim. Biophys. Acta 1078:388-394(1991).
CC   -!- FUNCTION: Catalyzes the first step in the metabolic activation of
CC       vitamin D(3) into 1-alpha,25-dihydroxyvitamin D(3), its active,
CC       hormonal form.
CC   -!- CATALYTIC ACTIVITY: 5-beta-cholestane-3-alpha,7-alpha,12-alpha-
CC       triol + NADPH + O(2) = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-
CC       alpha,26-tetraol + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- TISSUE SPECIFICITY: Found in liver and kidney.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; Y16417; CAA76205.1; -; mRNA.
DR   PIR; JC5819; JC5819.
DR   RefSeq; NP_999559.1; -.
DR   UniGene; Ssc.55051; -.
DR   HSSP; P00179; 1DT6.
DR   GeneID; 397687; -.
DR   KEGG; ssc:397687; -.
DR   HOVERGEN; O46658; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005792; C:microsome; IEA:UniProtKB-KW.
DR   GO; GO:0047749; F:cholestanetriol 26-monooxygenase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016712; F:oxidoreductase activity, acting on paired d...; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR008069; Cyt_P450_E_grp-I_CYP2D-like.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR01686; EP450ICYP2D.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endoplasmic reticulum; Heme; Iron;
KW   Membrane; Metal-binding; Microsome; Monooxygenase; NADP;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    500       Vitamin D(3) 25-hydroxylase.
FT                                /FTId=PRO_0000051745.
FT   METAL       446    446       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   500 AA;  56512 MW;  F1C878A02C44503D CRC64;
     MGLLTGDLLG ILALAMVIFL LLVDLMHRRS RWAPRYPPGP MPLPGLGNLL QVNFQDPRLS
     FIQLRRRFGD VFSLQQIWRP VVVLNGLAAV REALVSHSHE TSDRPPVFIL EHLGYGPRSE
     GVILARYGKA WREQRRFSVS TLRNFGLGKK SLEEWVTQEA SCLCAAFADQ AGRPFSPNNL
     LNKAVSNVIA SLTFARRFEY NDPRMLKLLD LVLEGLKEEV GLMRQVLEAM PVLRHIPGLC
     AKLFPRQKAF LVMIDELITE HKMTRDLAQP PRDLTDAFLD EMKEAKGNPE SSFNDENLRL
     VVAHLFSAGM ITTSTTLAWA LLLMILHPDV QRRVQQEIDE VIGHVRQPEI KDQALMPFTL
     AVLHEVQRFG DIVPLGVAHM TSCDIEVQGF LIPKGTTLIT NLTSVLKDET VWKKPFRFYP
     EHFLDAQGRF TKQEAFMPFS AGRRSCLGEP LARMELFLFF TTLLQAFSFS VPTGQPCPSD
     HGVFAFLLFP SPYQLCAVPR
//