[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-57; 249-273 AND 408-430. TISSUE=Liver; DOI=10.1006/bbrc.1997.7551; PubMed=9425298 [NCBI, ExPASy, EBI, Israel, Japan] Postlind H., Axen E., Bergman T., Wikvall K.; "Cloning, structure, and expression of a cDNA encoding vitamin D3 25-hydroxylase."; Biochem. Biophys. Res. Commun. 241:491-497(1997).
[2]	PROTEIN SEQUENCE OF 2-17. TISSUE=Liver; PubMed=1445236 [NCBI, ExPASy, EBI, Israel, Japan] Axen E., Bergman T., Wikvall K.; "Purification and characterization of a vitamin D3 25-hydroxylase from pig liver microsomes."; Biochem. J. 287:725-731(1992).
[3]	PROTEIN SEQUENCE OF 2-11. TISSUE=Liver; DOI=10.1016/0167-4838(91)90161-R; PubMed=1859829 [NCBI, ExPASy, EBI, Israel, Japan] Sono H., Sonoda Y., Sato Y.; "Purification and characterization of cytochrome P-45014DM (lanosterol 14 alpha-demethylase) from pig liver microsomes."; Biochim. Biophys. Acta 1078:388-394(1991).

Comments

FUNCTION: Catalyzes the first step in the metabolic activation of vitamin D(3) into 1-alpha,25-dihydroxyvitamin D(3), its active, hormonal form.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Found in liver and kidney.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Y16417; CAA76205.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

JC5819; JC5819.

NP_999559.1; -.

3D structure databases

HSSP

P00179; 1DT6. [HSSP ENTRY / PDB]

ModBase

O46658.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
IPR008069; Cyt_P450_E_grp-I_CYP2D-like.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR01686; EP450ICYP2D.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

O46658.

Genome annotation databases

GeneID

397687; -.

KEGG

ssc:397687; -.

Phylogenomic databases

HOVERGEN

O46658; -.

Other

ProtoNet

O46658.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 500`	`499`	`Cytochrome P450 2D25.`	`PRO_0000051745`
`METAL`	`446 446`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 500 AA [This is the length of the unprocessed precursor]

Molecular weight: 56512 Da [This is the MW of the unprocessed precursor]

CRC64: F1C878A02C44503D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGLLTGDLLG ILALAMVIFL LLVDLMHRRS RWAPRYPPGP MPLPGLGNLL QVNFQDPRLS 

        70         80         90        100        110        120 
FIQLRRRFGD VFSLQQIWRP VVVLNGLAAV REALVSHSHE TSDRPPVFIL EHLGYGPRSE 

       130        140        150        160        170        180 
GVILARYGKA WREQRRFSVS TLRNFGLGKK SLEEWVTQEA SCLCAAFADQ AGRPFSPNNL 

       190        200        210        220        230        240 
LNKAVSNVIA SLTFARRFEY NDPRMLKLLD LVLEGLKEEV GLMRQVLEAM PVLRHIPGLC 

       250        260        270        280        290        300 
AKLFPRQKAF LVMIDELITE HKMTRDLAQP PRDLTDAFLD EMKEAKGNPE SSFNDENLRL 

       310        320        330        340        350        360 
VVAHLFSAGM ITTSTTLAWA LLLMILHPDV QRRVQQEIDE VIGHVRQPEI KDQALMPFTL 

       370        380        390        400        410        420 
AVLHEVQRFG DIVPLGVAHM TSCDIEVQGF LIPKGTTLIT NLTSVLKDET VWKKPFRFYP 

       430        440        450        460        470        480 
EHFLDAQGRF TKQEAFMPFS AGRRSCLGEP LARMELFLFF TTLLQAFSFS VPTGQPCPSD 

       490        500 
HGVFAFLLFP SPYQLCAVPR

O46658 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools