NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/s002940050412; PubMed=9871122 [NCBI, ExPASy, EBI, Israel, Japan]
Skinner W., Bailey A.M., Renwick A., Keon J.P.R., Gurr S.J., Hargreaves J.A.;
"A single amino-acid substitution in the iron-sulphur protein subunit of succinate dehydrogenase determines resistance to carboxin in Mycosphaerella graminicola.";
Curr. Genet. 34:393-398(1998).

Comments

FUNCTION: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (By similarity).
CATALYTIC ACTIVITY: Succinate + ubiquinone = fumarate + ubiquinol.
COFACTOR: Binds 1 2Fe-2S cluster (By similarity).
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle .
SUBUNIT: Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit (By similarity).
SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side (By similarity).
SIMILARITY: Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
SIMILARITY: Contains 1 2Fe-2S ferredoxin-type domain.
SIMILARITY: Contains 1 4Fe-4S ferredoxin-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF042062; AAB97419.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P07014; 1NEK. [HSSP ENTRY / PDB]

SMR

O42772; 61-294.

ModBase

O42772.

Ontologies

GO:0005743;	Cellular component: mitochondrial inner membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0051537;	Molecular function: 2 iron, 2 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051538;	Molecular function: 3 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008177;	Molecular function: succinate dehydrogenase (ubiquinone) activity (inferred from electronic annotation from EC).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
GO:0006099;	Biological process: tricarboxylic acid cycle (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR006058; 2Fe2S_fd_BS.
IPR001450; 4Fe4S_Fe_S_bd.
IPR012675; b-grasp_ferredoxin-like.
IPR001041; Ferredoxin.
IPR012285; Fum_reductase_C.
IPR004489; Succ_DHase/fum_Rdtase_Fe-S.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.20.30; Ferredoxin_fold; 1.
G3DSA:1.10.1060.10; Fum_reductase_C; 1.

Pfam

PF00111; Fer2; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00384; dhsB; 1.

PROSITE

PS00197; 2FE2S_FER_1; 1.
PS51085; 2FE2S_FER_2; 1.
PS00198; 4FE4S_FER_1; 1.
PS51379; 4FE4S_FER_2; 1.
PROSITE graphical view of domain structure (profiles).

Other

ProtoNet

O42772.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; 3Fe-4S; 4Fe-4S; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide; Transport; Tricarboxylic acid cycle.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 297`		`Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial.`	`PRO_0000010349`
`DOMAIN`	`78 157`	`80`	`2Fe-2S ferredoxin-type.`
`DOMAIN`	`199 229`	`31`	`4Fe-4S ferredoxin-type.`
`METAL`	`117 117`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`122 122`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`125 125`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`137 137`		`Iron-sulfur 1 (2Fe-2S) (By similarity).`
`METAL`	`209 209`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`212 212`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`215 215`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`219 219`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`266 266`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`272 272`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`276 276`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`BINDING`	`224 224`		`Ubiquinone; shared with DHSD (By similarity).`

Sequence information

Length: 297 AA [This is the length of the unprocessed precursor]

Molecular weight: 33667 Da [This is the MW of the unprocessed precursor]

CRC64: A50010A24F48F6D4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MALRLATRRF APIAFRRGMA TTIEHTKEPI SATAEALSAS RPPIKETKTS TVKEPQMDAD 

        70         80         90        100        110        120 
AKTKTFHIYR WNPDQPTDKP RMQSYTLDLN KTGPMMLDAL IRIKNEVDPT LTFRRSCREG 

       130        140        150        160        170        180 
ICGSCAMNID GVNTLACLCR IPTDTAKETR IYPLPHTYVV KDLVPDMTQF YKQYKSIKPY 

       190        200        210        220        230        240 
LQRDTAPPDG KENRQSVADR KKLDGLYECI LCACCSTSCP SYWWNSEEYL GPAVLLQSYR 

       250        260        270        280        290 
WINDSRDEKT AQRKDALNNS MSLYRCHTIL NCSRTCPKGL NPALAIAEIK KSMAFTG

O42772 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools