ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O35132

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name CP27B_RAT
Primary accession number O35132
Secondary accession number O35076
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on July 15, 1998 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 60)
Name and origin of the protein
Protein name 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial [Precursor]
Synonyms EC 1.14.13.13
Cytochrome P450 subfamily XXVIIB polypeptide 1
Cytochrome p450 27B1
Calcidiol 1-monooxygenase
25-OHD-1 alpha-hydroxylase
25-hydroxyvitamin D(3) 1-alpha-hydroxylase
VD3 1A hydroxylase
P450C1 alpha
P450VD1-alpha
Gene name
Name: Cyp27b1
Synonyms: Cyp27b
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Sprague-Dawley;
PubMed=9333115 [NCBI, ExPASy, EBI, Israel, Japan]
St Arnaud R., Messerlian S., Moir J.M., Omdahl J.L., Glorieux F.H.;
"The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the pseudovitamin D-deficiency rickets (PDDR) disease locus.";
J. Bone Miner. Res. 12:1552-1559(1997).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Kidney;
DOI=10.1073/pnas.94.24.12920; PubMed=9371776 [NCBI, ExPASy, EBI, Israel, Japan]
Shinki T., Shimada H., Wakino S., Anazawa H., Hayashi M., Saruta T., Deluca H.F., Suda T.;
"Cloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA.";
Proc. Natl. Acad. Sci. U.S.A. 94:12920-12925(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF000139; AAB86461.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB001992; BAA23271.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_446215.1; -.
UniGene Rn.10847
3D structure databases
HSSP P00189; 1SCC. [HSSP ENTRY / PDB]
ModBase O35132.
Organism-specific databases
RGD 69192; Cyp27b1.
Ontologies
GO
GO:0031966; Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004498; Molecular function: calcidiol 1-monooxygenase activity (inferred from electronic annotation from EC).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
Genome annotation databases
GeneID 114700; -.
KEGG rno:114700; -.
Phylogenomic databases
HOVERGEN O35132; -.
Other
NextBio 618833; -.
ProtoNet O35132.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Heme; Iron; Membrane; Metal-binding; Mitochondrion; Monooxygenase; NADP; Oxidoreductase; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   501        25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial. PRO_0000003624
METAL   448   448        Iron (heme axial ligand) (By similarity). 
CONFLICT   13    13        H -> D (in Ref. 1; AAB86461). 
CONFLICT   55    55        H -> D (in Ref. 1; AAB86461). 
CONFLICT   103   112        FSSWSEHRRR -> SHLGQSTVAS (in Ref. 1; AAB86461). 
CONFLICT   119   119        L -> W (in Ref. 1; AAB86461). 
CONFLICT   129   144        RLRSLLAPLLLRPQAA -> EAPKSPGPASPPTSSS (in Ref. 1; AAB86461). 
CONFLICT   201   201        G -> R (in Ref. 1; AAB86461). 
CONFLICT   251   251        D -> N (in Ref. 1; AAB86461). 
CONFLICT   288   288        H -> D (in Ref. 1; AAB86461). 
CONFLICT   305   305        T -> R (in Ref. 1; AAB86461). 
CONFLICT   372   374        RLY -> MLD (in Ref. 1; AAB86461). 
Sequence information
Length: 501 AA [This is the length of the unprocessed precursor] Molecular weight: 55369 Da [This is the MW of the unprocessed precursor] CRC64: B0A85286A219EA0E [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTQAVKLASR VFHRVQLPSQ LGSDSVLRSL SDIPGPSTPS FLAELFCKGG LSRLHELQVH 

        70         80         90        100        110        120 
GAARYGPIWS GSFGTLRTVY VADPALVEQL LRQESHCPER CSFSSWSEHR RRHQRACGLL 

       130        140        150        160        170        180 
TADGEEWQRL RSLLAPLLLR PQAAAGYAGT LDSVVSDLVR RLRRQRGRGS GLPDLVLDVA 

       190        200        210        220        230        240 
GEFYKFGLEG IGAVLLGSRL GCLEAEVPPD TETFIEAVGS VFVSTLLTMA MPSWLHRLIP 

       250        260        270        280        290        300 
GPWARLCRDW DQMFAFAQKH VEQREGEAAV RNQGKPEEDL PTGHHLTHFL FREKVSVQSI 

       310        320        330        340        350        360 
VGNVTELLLA GVDTVSNTLS WALYELSRHP EVQSALHSEI TGAVNPGSYA HLQATALSQL 

       370        380        390        400        410        420 
PLLKAVIKEV LRLYPVVPGN SRVPDRDICV GNYVIPQDTL VSLCHYATSR DPAQFREPNS 

       430        440        450        460        470        480 
FNPARWLGEG PAPHPFASLP FGFGKRSCIG RRLAELELQM ALAQILTHFE VLPEPGALPV 

       490        500 
KPMTRTVLVP ERSIHLQFVD R
O35132 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by kr flag YPRC Korea Mirror sites: Australia Brazil Canada China Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!