NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 43-57, AND BIOPHYSICOCHEMICAL PROPERTIES.
STRAIN=ATCC 29267 / DSM 3873 / LMG 2657 / NCPPB 3004;
PubMed=9352901 [NCBI, ExPASy, EBI, Israel, Japan]
Yum D.-Y., Lee Y.-P., Pan J.-G.;
"Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli.";
J. Bacteriol. 179:6566-6572(1997).

Comments

FUNCTION: Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. The role of this subunit in the heterotrimer is not known.
CATALYTIC ACTIVITY: D-gluconate + acceptor = 2-dehydro-D-gluconate + reduced acceptor.
BIOPHYSICOCHEMICAL PROPERTIES:

pH dependence: Optimum pH is 5.0;
Temperature dependence: Optimum temperature is 30 degrees Celsius;
SUBUNIT: Heterotrimer.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Periplasmic side (Potential).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U97665; AAC45883.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A38575; A38575.

3D structure databases

ModBase

O34213.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0033717;	Molecular function: gluconate 2-dehydrogenase (acceptor) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

ProtoNet

O34213.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Direct protein sequencing; Membrane; Oxidoreductase; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 42`	`42`	`Tat-type signal.`
`CHAIN`	`43 220`	`178`	`Gluconate 2-dehydrogenase subunit 3.`	`PRO_0000045856`

Sequence information

Length: 220 AA [This is the length of the unprocessed precursor]

Molecular weight: 24368 Da [This is the MW of the unprocessed precursor]

CRC64: CDDB85B110663803 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSEHKNGHTR RDFLLRTITL APAMAVGSTA MGALVAPMAA GAAEQSSGSQ TARDYQPTWF 

        70         80         90        100        110        120 
TAEEFAFITA AVARLIPNDE RGPGALEAGV PEFIDRQMNT PYALGSNWYM QGPFNPDLPK 

       130        140        150        160        170        180 
ELGYQLPLVP QQIYRLGLAD ADSWSKHQHG KVFAELSGDQ QDALLSDFES GKAEFTQLPA 

       190        200        210        220 
KTFFSFLLQN TREGYFTRSD PRWQSGHGGL EADWLPRRTR

O34213 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools