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UniProtKB/Swiss-Prot entry O32141


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name URIC_BACSU
Primary accession number O32141
Secondary accession numbers None
Integrated into Swiss-Prot on July 15, 1999
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 60)
Name and origin of the protein
Protein name Uricase
Synonyms EC 1.7.3.3
Urate oxidase
Gene name
Name: pucL
Synonyms: yunL
OrderedLocusNames: BSU32450
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
[2]
FUNCTION.
STRAIN=168;
DOI=10.1128/JB.183.11.3293-3302.2001; PubMed=11344136 [NCBI, ExPASy, EBI, Israel, Japan]
Schultz A.C., Nygaard P., Saxild H.H.;
"Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.";
J. Bacteriol. 183:3293-3302(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z99120; CAB15235.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G70016; G70016.
RefSeq NP_391125.1; -.
3D structure databases
ModBase O32141.
Enzyme and pathway databases
BioCyc BSUB224308:BSU3242-MON; -.
Organism-specific databases
SubtiList BG13986; pucL. [Micado]
Ontologies
GO
GO:0004846; Molecular function: urate oxidase activity (inferred from electronic annotation from InterPro).
GO:0019428; Biological process: allantoin biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006144; Biological process: purine base metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR017580; OHCU_decarboxylase-1.
IPR002042; Uricase.
Graphical view of domain structure.
Gene3D G3DSA:3.10.270.10; Uricase; 1.
Pfam PF01014; Uricase; 2.
Pfam graphical view of domain structure.
PRINTS PR00093; URICASE.
ProDom PD003367; Uricase; 2.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR03383; urate_oxi; 1.
PROSITE PS00366; URICASE; 1.
Genome annotation databases
GeneID 936669; -.
GenomeReviews AL009126_GR; BSU32450.
KEGG bsu:BSU32450; -.
NMPDR fig|224308.1.peg.3251; -.
Phylogenomic databases
HOGENOM O32141; -.
Genome annotation databases
CMR O32141; BSU32450.
Other
ProtoNet O32141.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Oxidoreductase; Purine metabolism.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   494  494     Uricase. PRO_0000166006
ACT_SITE   366   366        Charge relay system (By similarity). 
ACT_SITE   415   415        Charge relay system (By similarity). 
Sequence information
Length: 494 AA [This is the length of the unprocessed precursor] Molecular weight: 56560 Da [This is the MW of the unprocessed precursor] CRC64: C16D0BB42F67BFC5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MFTMDDLNQM DTQTLTDTLG SIFEHSSWIA ERSAALRPFS SLSDLHRKMT GIVKAADRET 

        70         80         90        100        110        120 
QLDLIKKHPR LGTKKTMSDD SVREQQNAGL GKLEQQEYEE FLMLNEHYYD RFGFPFILAV 

       130        140        150        160        170        180 
KGKTKQDIHQ ALLARLESER ETEFQQALIE IYRIARFRLA DIITEKGETQ MKRTMSYGKG 

       190        200        210        220        230        240 
NVFAYRTYLK PLTGVKQIPE SSFAGRDNTV VGVDVTCEIG GEAFLPSFTD GDNTLVVATD 

       250        260        270        280        290        300 
SMKNFIQRHL ASYEGTTTEG FLHYVAHRFL DTYSHMDTIT LTGEDIPFEA MPAYEEKELS 

       310        320        330        340        350        360 
TSRLVFRRSR NERSRSVLKA ERSGNTITIT EQYSEIMDLQ LVKVSGNSFV GFIRDEYTTL 

       370        380        390        400        410        420 
PEDGNRPLFV YLNISWQYEN TNDSYASDPA RYVAAEQVRD LASTVFHELE TPSIQNLIYH 

       430        440        450        460        470        480 
IGCRILARFP QLTDVSFQSQ NHTWDTVVEE IPGSKGKVYT EPRPPYGFQH FTVTREDAEK 

       490 
EKQKAAEKCR SLKA 

O32141 in FASTA format

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