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UniProtKB/Swiss-Prot entry O24606


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name EIN3_ARATH
Primary accession number O24606
Secondary accession number Q8VYW4
Integrated into Swiss-Prot on September 26, 2003
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 61)
Name and origin of the protein
Protein name Protein ETHYLENE INSENSITIVE 3
Synonyms None
Gene name
Name: EIN3
OrderedLocusNames: At3g20770
ORFNames: MOE17.8
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND MUTANTS EIN3-1; EIN3-2 AND EIN3-3.
STRAIN=cv. Columbia;
DOI=10.1016/S0092-8674(00)80300-1; PubMed=9215635 [NCBI, ExPASy, EBI, Israel, Japan]
Chao Q., Rothenberg M., Solano R., Roman G., Terzaghi W., Ecker J.R.;
"Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins.";
Cell 89:1133-1144(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.2.131; PubMed=10819329 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:131-135(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
CHARACTERIZATION, AND FUNCTION.
PubMed=9851977 [NCBI, ExPASy, EBI, Israel, Japan]
Solano R., Stepanova A.N., Chao Q., Ecker J.R.;
"Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.";
Genes Dev. 12:3703-3714(1998).
Comments
  • FUNCTION: Probable transcription factor acting as a positive regulator in the ethylene response pathway. Is required for ethylene responsiveness in adult plant tissues. Binds a primary ethylene response element present in the ETHYLENE-RESPONSE-FACTOR1 promoter with consequence to activate the transcription of this gene.
  • SUBUNIT: Acts as homodimer to bind the primary ethylene response element.
  • INTERACTION:
    Q9SKK0:EBF1; NbExp=1; IntAct=EBI-593576, EBI-401198;
    Q708Y0:EBF2; NbExp=1; IntAct=EBI-593576, EBI-593623;
  • SUBCELLULAR LOCATION: Nucleus.
  • MISCELLANEOUS: Loss-of-function mutations (ein3-1 and ein3-2) in the gene lead to the suppression of ethylene-mediated effects including gene expression, the triple response, cell growth inhibition, and accelerated senescence.
  • SIMILARITY: Belongs to the EIN3 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF004216; AAC49749.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF004217; AAC49750.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB025629; BAB02485.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY069875; AAL47431.2; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_188713.1; -.
UniGene At.24824
3D structure databases
SMR O24606; 178-301.
ModBase O24606.
Protein-protein interaction databases
IntAct O24606; 2.
Organism-specific databases
GeneFarm 2364; -.
TAIR At3g20770; -.
Gene expression databases
ArrayExpress O24606; -.
GermOnline AT3G20770; Arabidopsis thaliana.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003700; Molecular function: transcription factor activity (inferred from direct assay from TAIR).
GO:0009873; Biological process: ethylene mediated signaling pathway (inferred from electronic annotation from UniProtKB-KW).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from UniProtKB-KW).
GO:0010182; Biological process: sugar mediated signaling (traceable author statement from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR006957; EIN3.
Graphical view of domain structure.
Pfam PF04873; EIN3; 1.
Pfam graphical view of domain structure.
Proteomics databases
PRIDE O24606; -.
Genome annotation databases
GeneID 821625; -.
GenomeReviews BA000014_GR; AT3G20770.
KEGG ath:AT3G20770; -.
NMPDR fig|3702.1.peg.14310; -.
Other
ProtoNet O24606.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Activator; Coiled coil; Complete proteome; DNA-binding; Ethylene signaling pathway; Nucleus; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   628  628     Protein ETHYLENE INSENSITIVE 3. PRO_0000113498
COILED   38    68  31     Potential. 
COMPBIAS   533   583  51     Asn-rich. 
MUTAGEN   245   245        K->N: In ein3-3; suppression of DNA-binding. 
Sequence information
Length: 628 AA [This is the length of the unprocessed precursor] Molecular weight: 71421 Da [This is the MW of the unprocessed precursor] CRC64: 8DA269C9F2A3BFD3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MMFNEMGMCG NMDFFSSGSL GEVDFCPVPQ AEPDSIVEDD YTDDEIDVDE LERRMWRDKM 

        70         80         90        100        110        120 
RLKRLKEQDK GKEGVDAAKQ RQSQEQARRK KMSRAQDGIL KYMLKMMEVC KAQGFVYGII 

       130        140        150        160        170        180 
PENGKPVTGA SDNLREWWKD KVRFDRNGPA AITKYQAENN IPGIHEGNNP IGPTPHTLQE 

       190        200        210        220        230        240 
LQDTTLGSLL SALMQHCDPP QRRFPLEKGV PPPWWPNGKE DWWPQLGLPK DQGPAPYKKP 

       250        260        270        280        290        300 
HDLKKAWKVG VLTAVIKHMF PDIAKIRKLV RQSKCLQDKM TAKESATWLA IINQEESLAR 

       310        320        330        340        350        360 
ELYPESCPPL SLSGGSCSLL MNDCSQYDVE GFEKESHYEV EELKPEKVMN SSNFGMVAKM 

       370        380        390        400        410        420 
HDFPVKEEVP AGNSEFMRKR KPNRDLNTIM DRTVFTCENL GCAHSEISRG FLDRNSRDNH 

       430        440        450        460        470        480 
QLACPHRDSR LPYGAAPSRF HVNEVKPVVG FPQPRPVNSV AQPIDLTGIV PEDGQKMISE 

       490        500        510        520        530        540 
LMSMYDRNVQ SNQTSMVMEN QSVSLLQPTV HNHQEHLQFP GNMVEGSFFE DLNIPNRANN 

       550        560        570        580        590        600 
NNSSNNQTFF QGNNNNNNVF KFDTADHNNF EAAHNNNNNS SGNRFQLVFD STPFDMASFD 

       610        620 
YRDDMSMPGV VGTMDGMQQK QQDVSIWF 

O24606 in FASTA format

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