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[1]
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NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
TISSUE=Prostate;
DOI=10.1074/jbc.272.25.15959; PubMed=9188497 [NCBI, ExPASy, EBI, Israel, Japan]
Biswas M.G.,
Russell D.W.;
"Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate.";
J. Biol. Chem. 272:15959-15966(1997).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
TISSUE=Liver;
DOI=10.1074/jbc.M000562200; PubMed=10896656 [NCBI, ExPASy, EBI, Israel, Japan]
Huang X.-F.,
Luu-The V.;
"Molecular characterization of a first human 3(alpha-->beta)-hydroxysteroid epimerase.";
J. Biol. Chem. 275:29452-29457(2000).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[4]
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FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
DOI=10.1006/abbi.2000.2203; PubMed=11360992 [NCBI, ExPASy, EBI, Israel, Japan]
Chetyrkin S.V.,
Hu J.,
Gough W.H.,
Dumaual N.,
Kedishvili N.Y.;
"Further characterization of human microsomal 3alpha-hydroxysteroid dehydrogenase.";
Arch. Biochem. Biophys. 386:1-10(2001).
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[5]
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FUNCTION.
DOI=10.1016/S0167-4781(01)00247-0; PubMed=11513953 [NCBI, ExPASy, EBI, Israel, Japan]
Huang X.-F.,
Luu-The V.;
"Gene structure, chromosomal localization and analysis of 3-ketosteroid reductase activity of the human 3(alpha-->beta)-hydroxysteroid epimerase.";
Biochim. Biophys. Acta 1520:124-130(2001).
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- FUNCTION: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha-androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids.
- CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
- CATALYTIC ACTIVITY: Testosterone + NAD+ = androst-4-ene-3,17-dione + NADH.
- CATALYTIC ACTIVITY: Retinol + NAD+ = retinal + NADH.
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
KM=0.19 µM for NAD; | | KM=0.18 µM for NADH; | | KM=54 µM for NADPH; | | KM=940 µM for NADP; | | KM=3.2 µM for all-trans-retinol; | | KM=0.24 µM for allopregnanolone; | | KM=0.13 µM for 3-alpha-androstanediol; | | KM=0.23 µM for androsterone; | | KM=0.13 µM for dehydroepiandrosterone; | | Vmax=1.2 nmol/min/mg enzyme with all-trans-retinol; | | Vmax=14.7 nmol/min/mg enzyme with allopregnanolone; | | Vmax=16.5 nmol/min/mg enzyme with 3-alpha-androstanediol; | | Vmax=35 nmol/min/mg enzyme with androsterone; | | Vmax=0.90 nmol/min/mg enzyme with dehydroepiandrosterone; | | Note=the kinetic parameters were determined using microsomes from transfected cells; | |
- SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein; Lumenal side. Early endosome membrane; Peripheral membrane protein; Lumenal side (Potential).
- TISSUE SPECIFICITY: Detected in liver and prostate (at protein level). Detected in adult liver, lung, brain, placenta, prostate, adrenal gland, testis, mammary gland, spleen, spinal cord and uterus. Detected in caudate nucleus, and at lower levels in amygdala, corpus callosum, hippocampus, substantia nigra and thalamus. Detected in fetal lung, liver and brain.
- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
- SEQUENCE CAUTION:
- Sequence=AAB88252.1; Type=Frameshift; Positions=158, 174;
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 317 AA [This is the length of the unprocessed precursor] |
Molecular weight: 35966 Da [This is the MW of the unprocessed precursor] |
CRC64: 46F1E940605CBEE9 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MWLYLAAFVG LYYLLHWYRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD ARGLRVLAAC
70 80 90 100 110 120
LTEKGAEQLR GQTSDRLETV TLDVTKMESI AAATQWVKEH VGDRGLWGLV NNAGILTPIT
130 140 150 160 170 180
LCEWLNTEDS MNMLKVNLIG VIQVTLSMLP LVRRARGRIV NVSSILGRVA FFVGGYCVSK
190 200 210 220 230 240
YGVEAFSDIL RREIQHFGVK ISIVEPGYFR TGMTNMTQSL ERMKQSWKEA PKHIKETYGQ
250 260 270 280 290 300
QYFDALYNIM KEGLLNCSTN LNLVTDCMEH ALTSVHPRTR YSAGWDAKFF FIPLSYLPTS
310
LADYILTRSW PKPAQAV
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O14756 in FASTA format |
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