ID   DCTN1_MOUSE             Reviewed;        1281 AA.
AC   O08788; Q3TZG7;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   22-JUL-2008, entry version 58.
DE   RecName: Full=Dynactin subunit 1;
DE   AltName: Full=150 kDa dynein-associated polypeptide;
DE   AltName: Full=DAP-150;
DE            Short=DP-150;
DE   AltName: Full=p150-glued;
GN   Name=Dctn1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J;
RX   MEDLINE=97223454; PubMed=9070275; DOI=10.1006/bbrc.1997.6095;
RA   Jang W., Weber J.S., Tokito M.K., Holzbaur E.L., Meisler M.H.;
RT   "Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a
RT   candidate for the neuromuscular disease mutation mnd2.";
RL   Biochem. Biophys. Res. Commun. 231:344-347(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Inner ear;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=17203969; DOI=10.1021/pr0604155;
RA   Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
RT   "Protein phosphorylation and expression profiling by Yin-yang
RT   multidimensional liquid chromatography (Yin-yang MDLC) mass
RT   spectrometry.";
RL   J. Proteome Res. 6:250-262(2007).
CC   -!- FUNCTION: Required for the cytoplasmic dynein-driven retrograde
CC       movement of vesicles and organelles along microtubules. Dynein-
CC       dynactin interaction is a key component of the mechanism of axonal
CC       transport of vesicles and organelles.
CC   -!- SUBUNIT: Large macromolecular complex of at least 10 components;
CC       p150(glued) binds directly to microtubules and to cytoplasmic
CC       dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O08788-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O08788-2; Sequence=VSP_029584;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the dynactin 150 kDa subunit family.
CC   -!- SIMILARITY: Contains 1 CAP-Gly domain.
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DR   EMBL; U60312; AAB57773.1; -; mRNA.
DR   EMBL; AK157867; BAE34241.1; -; mRNA.
DR   PIR; JC5368; JC5368.
DR   UniGene; Mm.6919; -.
DR   HSSP; Q20728; 1LPL.
DR   SMR; O08788; 1-104.
DR   IntAct; O08788; -.
DR   PhosphoSite; O08788; -.
DR   Ensembl; ENSMUSG00000031865; Mus musculus.
DR   MGI; MGI:107745; Dctn1.
DR   HOGENOM; O08788; -.
DR   HOVERGEN; O08788; -.
DR   ArrayExpress; O08788; -.
DR   CleanEx; MM_DCTN1; -.
DR   GermOnline; ENSMUSG00000031865; Mus musculus.
DR   GO; GO:0005515; F:protein binding; IPI:MGI.
DR   InterPro; IPR000938; Cytoskel-assoc-prot_CAP-Gly.
DR   Pfam; PF01302; CAP_GLY; 1.
DR   PROSITE; PS00845; CAP_GLY_1; 1.
DR   PROSITE; PS50245; CAP_GLY_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Dynein;
KW   Microtubule; Motor protein; Phosphoprotein.
FT   CHAIN         1   1281       Dynactin subunit 1.
FT                                /FTId=PRO_0000083519.
FT   DOMAIN       48     90       CAP-Gly.
FT   COILED      214    547       Potential.
FT   COILED      943   1049       Potential.
FT   COILED     1185   1214       Potential.
FT   COMPBIAS    157    184       Ser-rich.
FT   MOD_RES     541    541       Phosphoserine.
FT   VAR_SEQ    1049   1086       Missing (in isoform 2).
FT                                /FTId=VSP_029584.
FT   CONFLICT    406    406       W -> R (in Ref. 1; AAB57773).
FT   CONFLICT    721    723       TKA -> NKG (in Ref. 1; AAB57773).
FT   CONFLICT    732    732       S -> R (in Ref. 1; AAB57773).
FT   CONFLICT   1202   1202       V -> I (in Ref. 1; AAB57773).
SQ   SEQUENCE   1281 AA;  141692 MW;  7DE78A105DE38C4C CRC64;
     MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV
     ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE
     GADAAAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGPSSSLGPS GSASAGELSS
     SEPSTPAQTP LAAPIIPTPA LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE
     DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT
     ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL
     KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVWQQRE RLQEELSQAE
     STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE
     TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA
     SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG
     GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL SFAAGLVYSL
     SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL
     TKAIKYYQHL YSIHLAEQPE DSTMQLADHI KFTQSALDCM GVEVGRLRAF LQGGQEATDI
     ALLLRDLETS CSDTRQFCKK IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV
     LQEVAAAAAQ LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM
     NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK
     IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE TQTLLRKKEK DFEETMDALQ
     ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG PPPSGIATLV SGIAGEEPQR GGAPGQAPGA
     LPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE
     GPGGNLVAGA LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD
     TVEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK VTFSCAAGLG
     QRHRLVLTQE QLHQLHSRLI S
//