[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). STRAIN=C57BL/6J; DOI=10.1006/bbrc.1997.6095; PubMed=9070275 [NCBI, ExPASy, EBI, Israel, Japan] Jang W., Weber J.S., Tokito M.K., Holzbaur E.L., Meisler M.H.; "Mouse p150Glued (dynactin 1) cDNA sequence and evaluation as a candidate for the neuromuscular disease mutation mnd2."; Biochem. Biophys. Res. Commun. 231:344-347(1997).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). STRAIN=C57BL/6J; TISSUE=Inner ear; DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan] Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; "The transcriptional landscape of the mammalian genome."; Science 309:1559-1563(2005).
[3]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541, AND MASS SPECTROMETRY. TISSUE=Liver; DOI=10.1021/pr0604155; PubMed=17203969 [NCBI, ExPASy, EBI, Israel, Japan] Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."; J. Proteome Res. 6:250-262(2007).

Comments

FUNCTION: Required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles.
SUBUNIT: Large macromolecular complex of at least 10 components; p150(glued) binds directly to microtubules and to cytoplasmic dynein. Interacts with the C-terminus of MAPRE1, MAPRE2 and MAPRE3 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	O08788-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	O08788-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_029584.

SIMILARITY: Belongs to the dynactin 150 kDa subunit family.
SIMILARITY: Contains 1 CAP-Gly domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U60312; AAB57773.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AK157867; BAE34241.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JC5368; JC5368.

UniGene

Mm.6919

3D structure databases

HSSP

Q20728; 1LPL. [HSSP ENTRY / PDB]

SMR

O08788; 1-104.

ModBase

O08788.

Protein-protein interaction databases

IntAct

O08788; -.

PTM databases

PhosphoSite

O08788; -.

Organism-specific databases

MGI

MGI:107745; Dctn1.

GeneLynx

Dctn1; Mus musculus.

Gene expression databases

ArrayExpress

O08788; -.

CleanEx

MM_DCTN1; -.

GermOnline

ENSMUSG00000031865; Mus musculus.

Ontologies

GO:0005515;	Molecular function: protein binding (inferred from physical interaction from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR000938; Cytoskel-assoc-prot_CAP-Gly.
Graphical view of domain structure.

Pfam

PF01302; CAP_GLY; 1.
Pfam graphical view of domain structure.

PROSITE

PS00845; CAP_GLY_1; 1.
PS50245; CAP_GLY_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

O08788.

Genome annotation databases

Ensembl

ENSMUSG00000031865; Mus musculus. [Contig view]

Phylogenomic databases

HOGENOM

O08788; -.

HOVERGEN

O08788; -.

Other

Dctn1; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Coiled coil; Cytoplasm; Cytoskeleton; Dynein; Microtubule; Motor protein; Phosphoprotein.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1281`	`1281`	`Dynactin subunit 1.`	`PRO_0000083519`
`DOMAIN`	`48 90`	`43`	`CAP-Gly.`
`COILED`	`214 547`	`334`	`Potential.`
`COILED`	`943 1049`	`107`	`Potential.`
`COILED`	`1185 1214`	`30`	`Potential.`
`COMPBIAS`	`157 184`	`28`	`Ser-rich.`
`MOD_RES`	`541 541`		`Phosphoserine.`
`VAR_SEQ`	`1049 1086`		`Missing (in isoform 2).`	`VSP_029584`
`CONFLICT`	`406 406`		`W -> R (in Ref. 1; AAB57773).`
`CONFLICT`	`721 723`		`TKA -> NKG (in Ref. 1; AAB57773).`
`CONFLICT`	`732 732`		`S -> R (in Ref. 1; AAB57773).`
`CONFLICT`	`1202 1202`		`V -> I (in Ref. 1; AAB57773).`

Sequence information

Length: 1281 AA [This is the length of the unprocessed precursor]

Molecular weight: 141692 Da [This is the MW of the unprocessed precursor]

CRC64: 7DE78A105DE38C4C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAQSRRHMSS RTPSGSRMST EASARPLRVG SRVEVIGKGH RGTVAYVGAT LFATGKWVGV 

        70         80         90        100        110        120 
ILDEAKGKND GTVQGRKYFT CDEGHGIFVR QSQIQVFEDG ADTTSPETPD SSASKVLKRE 

       130        140        150        160        170        180 
GADAAAKTSK LRGLKPKKAP TARKTTTRRP KPTRPASTGV AGPSSSLGPS GSASAGELSS 

       190        200        210        220        230        240 
SEPSTPAQTP LAAPIIPTPA LTSPGAAPPL PSPSKEEEGL RAQVRDLEEK LETLRLKRSE 

       250        260        270        280        290        300 
DKAKLKELEK HKIQLEQVQE WKSKMQEQQA DLQRRLKEAR KEAKEALEAK ERYMEEMADT 

       310        320        330        340        350        360 
ADAIEMATLD KEMAEERAES LQQEVEALKE RVDELTTDLE ILKAEIEEKG SDGAASSYQL 

       370        380        390        400        410        420 
KQLEEQNARL KDALVRMRDL SSSEKQEHVK LQKLMEKKNQ ELEVVWQQRE RLQEELSQAE 

       430        440        450        460        470        480 
STIDELKEQV DAALGAEEMV EMLTDRNLNL EEKVRELRET VGDLEAMNEM NDELQENARE 

       490        500        510        520        530        540 
TELELREQLD MAGARVREAQ KRVEAAQETV ADYQQTIKKY RQLTAHLQDV NRELTNQQEA 

       550        560        570        580        590        600 
SVERQQQPPP ETFDFKIKFA ETKAHAKAIE MELRQMEVAQ ANRHMSLLTA FMPDSFLRPG 

       610        620        630        640        650        660 
GDHDCVLVLL LMPRLICKAE LIRKQAQEKF DLSENCSERP GLRGAAGEQL SFAAGLVYSL 

       670        680        690        700        710        720 
SLLQATLHRY EHALSQCSVD VYKKVGSLYP EMSAHERSLD FLIELLHKDQ LDETVNVEPL 

       730        740        750        760        770        780 
TKAIKYYQHL YSIHLAEQPE DSTMQLADHI KFTQSALDCM GVEVGRLRAF LQGGQEATDI 

       790        800        810        820        830        840 
ALLLRDLETS CSDTRQFCKK IRRRMPGTDA PGIPAALAFG SQVSDTLLDC RKHLTWVVAV 

       850        860        870        880        890        900 
LQEVAAAAAQ LIAPLAENEG LPVAALEELA FKASEQIYGS PSSSPYECLR QSCTILISTM 

       910        920        930        940        950        960 
NKLATAMQEG EYDAERPPSK PPPVELRAAA LRAEITDAEG LGLKLEDRET VIKELKKSLK 

       970        980        990       1000       1010       1020 
IKGEELSEAN VRLSLLEKKL DSAAKDADER IEKVQTRLDE TQTLLRKKEK DFEETMDALQ 

      1030       1040       1050       1060       1070       1080 
ADIDQLEAEK AELKQRLNSQ SKRTIEGLRG PPPSGIATLV SGIAGEEPQR GGAPGQAPGA 

      1090       1100       1110       1120       1130       1140 
LPGPGLVKDS PLLLQQISAM RLHISQLQHE NSILRGAQMK ASLAALPPLH VAKLSLPPHE 

      1150       1160       1170       1180       1190       1200 
GPGGNLVAGA LYRKTSQLLE KLNQLSTHTH VVDITRSSPA AKSPSAQLME QVAQLKSLSD 

      1210       1220       1230       1240       1250       1260 
TVEKLKDEVL KETVTQRPGA TVPTDFATFP SSAFLRAKEE QQDDTVYMGK VTFSCAAGLG 

      1270       1280 
QRHRLVLTQE QLHQLHSRLI S

O08788 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools