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UniProtKB/Swiss-Prot entry O08547


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SC22B_MOUSE
Primary accession number O08547
Secondary accession number Q91VU3
Integrated into Swiss-Prot on March 29, 2004
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    December 16, 2008 (Entry version 81)
Name and origin of the protein
Protein name Vesicle-trafficking protein SEC22b
Synonyms SEC22 vesicle-trafficking protein homolog B
SEC22 vesicle-trafficking protein-like 1
mSec22b
ERS24
ERS-24
Gene name
Name: Sec22b
Synonyms: Sec22l1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=C57BL/6J;
TISSUE=Placenta;
DOI=10.1016/S0092-8674(00)80191-9; PubMed=9094723 [NCBI, ExPASy, EBI, Israel, Japan]
Hay J.C., Chao D.S., Kuo C.S., Scheller R.H.;
"Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells.";
Cell 89:149-158(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J, and NOD;
TISSUE=Embryo, Pituitary, and Thymus;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Mammary gland;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
PROTEIN SEQUENCE OF 29-38, AND MASS SPECTROMETRY.
STRAIN=C57BL/6;
TISSUE=Brain;
Lubec G., Kang S.U.;
Submitted (APR-2007) to UniProtKB.
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND MASS SPECTROMETRY.
TISSUE=Brain cortex;
DOI=10.1074/mcp.M600046-MCP200; PubMed=17114649 [NCBI, ExPASy, EBI, Israel, Japan]
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.";
Mol. Cell. Proteomics 6:283-293(2007).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1073/pnas.0609836104; PubMed=17242355 [NCBI, ExPASy, EBI, Israel, Japan]
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
"Large-scale phosphorylation analysis of mouse liver.";
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1021/pr800223m; PubMed=18630941 [NCBI, ExPASy, EBI, Israel, Japan]
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.;
"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis.";
J. Proteome Res. 7:3957-3967(2008).
[8]
X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-130.
DOI=10.1074/jbc.M101584200; PubMed=11309394 [NCBI, ExPASy, EBI, Israel, Japan]
Gonzalez L.C. Jr., Weis W.I., Scheller R.H.;
"A novel SNARE N-terminal domain revealed by the crystal structure of Sec22b.";
J. Biol. Chem. 276:24203-24211(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U91538; AAC53130.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK017237; BAB30646.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK034973; BAC28899.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK037918; BAC29901.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK088514; BAC40397.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK089928; BAC40999.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC009024; AAH09024.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_035472.1; -.
UniGene Mm.2551
3D structure databases
PDB
1IFQ; X-ray; 2.40 A; A/B=1-127.[ExPASy / RCSB / EBI]
PDBsum 1IFQ; -.
ModBase O08547.
PTM databases
PhosphoSite O08547; -.
Organism-specific databases
MGI MGI:1338759; Sec22b.
Gene expression databases
ArrayExpress O08547; -.
GermOnline ENSMUSG00000027879; Mus musculus.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from direct assay from MGI).
GO:0033116; Cellular component: ER-Golgi intermediate compartment membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0000139; Cellular component: Golgi membrane (inferred from direct assay from MGI).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0042470; Cellular component: melanosome (inferred from electronic annotation from UniProtKB-SubCell).
GO:0006888; Biological process: ER to Golgi vesicle-mediated transport (traceable author statement from MGI).
GO:0015031; Biological process: protein transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR010908; Longin.
IPR001388; Synaptobrevin.
Graphical view of domain structure.
Pfam PF00957; Synaptobrevin; 1.
Pfam graphical view of domain structure.
PRINTS PR00219; SYNAPTOBREVN.
PROSITE PS50859; LONGIN; 1.
PS50892; V_SNARE; 1.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE O08547; -.
Genome annotation databases
Ensembl ENSMUSG00000027879; Mus musculus. [Contig view]
GeneID 20333; -.
KEGG mmu:20333; -.
Phylogenomic databases
HOVERGEN O08547; -.
Other
NextBio 298135; -.
SOURCE Sec22b; Mus musculus.
ProtoNet O08547.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Coiled coil; Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   215  214     Vesicle-trafficking protein SEC22b. PRO_0000206771
TOPO_DOM   2   194  193     Cytoplasmic (Potential). 
TRANSMEM   195   215  21     Anchor for type IV membrane protein (Potential). 
DOMAIN   6   119  114     Longin. 
DOMAIN   134   194  61     v-SNARE coiled-coil homology. 
MOD_RES   35    35        Phosphoserine (By similarity). 
MOD_RES   48    48        Phosphoserine (By similarity). 
MOD_RES   137   137        Phosphoserine. 
MOD_RES   168   168        Phosphoserine. 
CONFLICT   189   189        M -> I (in Ref. 3; AAH09024). 
STRAND   4     9  6      
TURN   10    12  3      
STRAND   15    19  5      
HELIX   27    41  15      
STRAND   50    56  7      
STRAND   59    66  8      
STRAND   69    76  8      
HELIX   81    99  19      
TURN   100   105  6      
TURN   109   112  4      
HELIX   113   115  3      
HELIX   116   126  11      
Sequence information
Length: 215 AA [This is the length of the unprocessed precursor] Molecular weight: 24741 Da [This is the MW of the unprocessed precursor] CRC64: 29B4C55961C5A044 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT RCTLEAGAMT 

        70         80         90        100        110        120 
FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG KKVPTVSRPY SFIEFDTFIQ 

       130        140        150        160        170        180 
KTKKLYIDSR ARRNLGSINT ELQDVQRIMV ANIEEVLQRG EALSALDSKA NNLSSLSKKY 

       190        200        210 
RQDAKYLNMR STYAKLAAVA VFFIMLIVYV RFWWL 

O08547 in FASTA format

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