FUNCTION: Catalyzes the 3'5'-hydroxylation of naringenin and eriodictyol to form 5,7,3,'4',5'-pentahydroxyflavanone and 3',5'-hydroxylation of dihydrokaempferol and dihydroquercetin to form dihydromyricetin (By similarity).
CATALYTIC ACTIVITY: A flavanone + NADPH + O₂ = a 3'-hydroxyflavanone + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: A 3'-hydroxyflavanone + NADPH + O₂ = a 3',5'-dihydroxyflavanone + NADP⁺ + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Pigment biosynthesis; anthocyanin biosynthesis .
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

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Cross-references

Sequence databases

EMBL

D14590; BAA03440.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

O04773.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0033772;	Molecular function: flavonoid 3',5'-hydroxylase activity (inferred from electronic annotation from EC).
GO:0020037;	Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

ProtoNet

O04773.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Heme; Iron; Metal-binding; Monooxygenase; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 523`	`523`	`Flavonoid 3',5'-hydroxylase.`	`PRO_0000052135`
`METAL`	`460 460`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 523 AA [This is the length of the unprocessed precursor]

Molecular weight: 58726 Da [This is the MW of the unprocessed precursor]

CRC64: 70E39C6B76F387D9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSIDISTLFY ELVAAISLYL ATYSFIRFLF KPSHHHHLPP GPTGWPIIGA LPLLGTMPHV 

        70         80         90        100        110        120 
SLADMAVKYG PIMYLKLGSK GTVVASNPKA ARAFLKTHDA NFSNRPIDGG PTYLAYNAQD 

       130        140        150        160        170        180 
MVFAEYGPKW KLLRKLCSLH MLGPKALEDW AHVKVSEVGH MLKEMYEQSS KSVPVPVVVP 

       190        200        210        220        230        240 
EMLTYAMANM IGRIILSRRP FVITSKLDSS ASASASVSEF QYMVMELMRM AGLFNIGDFI 

       250        260        270        280        290        300 
PYIAWMDLQG IQRDMKVIQK KFDVLLNKMI KEHTESAHDR KDNPDFLDIL MAATQENTEG 

       310        320        330        340        350        360 
IQLNLVNVKA LLLDLFTAGT DTSSSVIEWA LAEMLNHRQI LNRAHEEMDQ VIGRNRRLEQ 

       370        380        390        400        410        420 
SDIPNLPYFQ AICKETFRKH PSTPLNLPRI STEACEVDGF HIPKNTRLIV NIWAIGRDPK 

       430        440        450        460        470        480 
VWENPLDFTP ERFLSEKHAK IDPRGNHFEL IPFGAGRRIC AGARMGAASV EYILGTLVHS 

       490        500        510        520 
FDWKLPDGVV EVNMEESFGI ALQKKVPLSA IVTPRLPPSS YTV

O04773 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools