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UniProtKB/Swiss-Prot entry O04197


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name COI1_ARATH
Primary accession number O04197
Secondary accession numbers None
Integrated into Swiss-Prot on February 15, 2005
Sequence was last modified on July 1, 1997 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 69)
Name and origin of the protein
Protein name Coronatine-insensitive protein 1
Synonyms F-box/LRR-repeat protein 2
AtFBL2
AtCOI1
COI-1
Gene name
Name: COI1
Synonyms: FBL2
OrderedLocusNames: At2g39940
ORFNames: T28M21.10
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
STRAIN=cv. Columbia;
DOI=10.1126/science.280.5366.1091; PubMed=9582125 [NCBI, ExPASy, EBI, Israel, Japan]
Xie D., Feys B.F., James S., Nieto-Rostro M., Turner J.G.;
"COI1: an Arabidopsis gene required for jasmonate-regulated defense and fertility.";
Science 280:1091-1094(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
FUNCTION.
STRAIN=cv. Columbia;
DOI=10.1105/tpc.6.5.751; PubMed=12244256 [NCBI, ExPASy, EBI, Israel, Japan]
Feys B.J.F., Benedetti C.S., Penfold C.N., Turner J.G.;
"Arabidopsis mutants selected for resistance to the phytotoxin coronatine are male sterile, insensitive to methyl jasmonate, and resistant to a bacterial pathogen.";
Plant Cell 6:751-759(1994).
[5]
FUNCTION.
DOI=10.1105/tpc.12.5.707; PubMed=10810145 [NCBI, ExPASy, EBI, Israel, Japan]
Reymond P., Weber H., Damond M., Farmer E.E.;
"Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis.";
Plant Cell 12:707-720(2000).
[6]
GENE FAMILY, AND NOMENCLATURE.
DOI=10.1016/S1360-1385(00)01769-6; PubMed=11077244 [NCBI, ExPASy, EBI, Israel, Japan]
Xiao W., Jang J.-C.;
"F-box proteins in Arabidopsis.";
Trends Plant Sci. 5:454-457(2000).
[7]
FUNCTION, AND MUTAGENESIS OF LEU-245.
DOI=10.1007/s00425-002-0787-4; PubMed=12172836 [NCBI, ExPASy, EBI, Israel, Japan]
Ellis C., Turner J.G.;
"A conditionally fertile coi1 allele indicates cross-talk between plant hormone signalling pathways in Arabidopsis thaliana seeds and young seedlings.";
Planta 215:549-556(2002).
[8]
FUNCTION, SUBUNIT, AND MUTAGENESIS OF GLU-22.
DOI=10.1105/tpc.003368; PubMed=12172031 [NCBI, ExPASy, EBI, Israel, Japan]
Xu L., Liu F., Lechner E., Genschik P., Crosby W.L., Ma H., Peng W., Huang D., Xie D.;
"The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis.";
Plant Cell 14:1919-1935(2002).
[9]
FUNCTION, SUBUNIT, AND MUTAGENESIS OF LEU-11; TRP-44 AND LEU-245.
DOI=10.1046/j.1365-313X.2002.01432.x; PubMed=12445118 [NCBI, ExPASy, EBI, Israel, Japan]
Devoto A., Nieto-Rostro M., Xie D., Ellis C., Harmston R., Patrick E., Davis J., Sherratt L., Coleman M., Turner J.G.;
"COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis.";
Plant J. 32:457-466(2002).
[10]
DOMAIN LEUCINE-RICH REPEATS.
DOI=10.1023/A:1014440531842; PubMed=12008900 [NCBI, ExPASy, EBI, Israel, Japan]
Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.;
"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression.";
Plant Mol. Biol. 49:69-79(2002).
[11]
FUNCTION, AND SUBUNIT.
DOI=10.1105/tpc.010207; PubMed=12724535 [NCBI, ExPASy, EBI, Israel, Japan]
Feng S., Ma L., Wang X., Xie D., Dinesh-Kumar S.P., Wei N., Deng X.W.;
"The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses.";
Plant Cell 15:1083-1094(2003).
[12]
FUNCTION.
DOI=10.1104/pp.103.022186; PubMed=12805591 [NCBI, ExPASy, EBI, Israel, Japan]
van Wees S.C.M., Chang H.-S., Zhu T., Glazebrook J.;
"Characterization of the early response of Arabidopsis to Alternaria brassicicola infection using expression profiling.";
Plant Physiol. 132:606-617(2003).
[13]
FUNCTION.
DOI=10.1111/j.1365-313X.2003.01986.x; PubMed=14756769 [NCBI, ExPASy, EBI, Israel, Japan]
He P., Chintamanani S., Chen Z., Zhu L., Kunkel B.N., Alfano J.R., Tang X., Zhou J.-M.;
"Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine.";
Plant J. 37:589-602(2004).
Comments
  • FUNCTION: Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola.
  • PATHWAY: Protein modification; protein ubiquitination.
  • SUBUNIT: Component of SCF(COI1) E3 ubiquitin ligase complexes at least composed of ASK1 or ASK2, CUL1, RBX1A or RBX1B and COI1. Interacts with ASK1 and ASK2, but separately. Interacts with RBCS-1B and HDA6. SCF complexes interact with the COP9 signalosome (CSN).
  • INTERACTION:
    Q94AH6:CUL1; NbExp=2; IntAct=EBI-401159, EBI-532411;
    Q9FML2:HDA6; NbExp=3; IntAct=EBI-401159, EBI-639608;
    P10798:RBCS-3B; NbExp=2; IntAct=EBI-401159, EBI-639640;
    Q39255:SKP1A; NbExp=6; IntAct=EBI-401159, EBI-532357;
    Q9FHW7:SKP1B; NbExp=5; IntAct=EBI-401159, EBI-604076;
    Q9LVI4:TIFY6B; NbExp=1; IntAct=EBI-401159, EBI-1792431;
  • DOMAIN: The F-box domain is essential for the formation of SFC(COI1) complexes.
  • DOMAIN: The Leu-rich domain is involved in the interactions with RBCS-1B and RPD3B.
  • MISCELLANEOUS: Mutants coi1-1 to coi1-14 are male sterile, insensitive to MeJA and coronatine, and exhibit enhanced resistance to Pseudomonas syringae atropurpurea (coronatine producing strain). Mutant coi1-16 has reduced sensitivity to jasmonate, but is male fertile when grown below 22 degrees Celsius and male sterile otherwise.
  • SIMILARITY: Contains 1 F-box domain.
  • SIMILARITY: Contains 16 LRR (leucine-rich) repeats.
  • WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants; URL="http://plantsubq.genomics.purdue.edu/";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF036340; AAC17498.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF002109; AAB95279.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY045625; AAK73983.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY133556; AAM91386.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T52139; T52139.
RefSeq NP_565919.1; -.
UniGene At.71018
3D structure databases
ModBase O04197.
Protein-protein interaction databases
IntAct O04197; 17.
Organism-specific databases
GeneFarm 4758; -.
TAIR At2g39940; -.
Gene expression databases
ArrayExpress O04197; -.
GermOnline AT2G39940; Arabidopsis thaliana.
Ontologies
GO
GO:0019005; Cellular component: SCF ubiquitin ligase complex (inferred from physical interaction from TAIR).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0042742; Biological process: defense response to bacterium (inferred from mutant phenotype from TAIR).
GO:0050832; Biological process: defense response to fungus (inferred from mutant phenotype from TAIR).
GO:0009861; Biological process: jasmonic acid and ethylene-dependent systemic resistance (traceable author statement from TAIR).
GO:0009867; Biological process: jasmonic acid mediated signaling pathway (inferred from genetic interaction from TAIR).
GO:0031348; Biological process: negative regulation of defense response (inferred from mutant phenotype from TAIR).
GO:0009625; Biological process: response to insect (inferred from mutant phenotype from TAIR).
GO:0031146; Biological process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (inferred from mutant phenotype from TAIR).
GO:0010118; Biological process: stomatal movement (inferred from mutant phenotype from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR001810; F-box.
Graphical view of domain structure.
PROSITE PS50181; FBOX; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).
Proteomics databases
PRIDE O04197; -.
Genome annotation databases
GeneID 818581; -.
GenomeReviews CT485783_GR; AT2G39940.
KEGG ath:AT2G39940; -.
NMPDR fig|3702.1.peg.11117; -.
Other
ProtoNet O04197.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Leucine-rich repeat; Plant defense; Repeat; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   592  592     Coronatine-insensitive protein 1. PRO_0000119960
DOMAIN   16    57  42     F-box. 
REPEAT   103   120  18     LRR 1. 
REPEAT   121   154  34     LRR 2. 
REPEAT   155   182  28     LRR 3. 
REPEAT   183   210  28     LRR 4. 
REPEAT   211   236  26     LRR 5. 
REPEAT   237   264  28     LRR 6. 
REPEAT   265   283  19     LRR 7. 
REPEAT   284   308  25     LRR 8. 
REPEAT   309   332  24     LRR 9. 
REPEAT   333   368  36     LRR 10. 
REPEAT   369   393  25     LRR 11. 
REPEAT   394   426  33     LRR 12. 
REPEAT   427   456  30     LRR 13. 
REPEAT   457   478  22     LRR 14. 
REPEAT   479   500  22     LRR 15. 
REPEAT   501   524  24     LRR 16. 
COMPBIAS   102   517  416     Leu-rich. 
MUTAGEN   11    11        L->A: No effects on interactions. 
MUTAGEN   22    22        E->A: Abrogates SFC(COI1) complexes formation, loss of response to jasmonate. 
MUTAGEN   44    44        W->A: Abrogates SFC(COI1) complexes formation and of interactions with RBCS-1B and RPD3B, loss of response to jasmonate. 
MUTAGEN   245   245        L->F: In coi1-16; abrogates interactions with RBCS-1B and RPD3B (coi1-16). 
Sequence information
Length: 592 AA [This is the length of the unprocessed precursor] Molecular weight: 67665 Da [This is the MW of the unprocessed precursor] CRC64: 1DDCF04990144C06 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEDPDIKRCK LSCVATVDDV IEQVMTYITD PKDRDSASLV CRRWFKIDSE TREHVTMALC 

        70         80         90        100        110        120 
YTATPDRLSR RFPNLRSLKL KGKPRAAMFN LIPENWGGYV TPWVTEISNN LRQLKSVHFR 

       130        140        150        160        170        180 
RMIVSDLDLD RLAKARADDL ETLKLDKCSG FTTDGLLSIV THCRKIKTLL MEESSFSEKD 

       190        200        210        220        230        240 
GKWLHELAQH NTSLEVLNFY MTEFAKISPK DLETIARNCR SLVSVKVGDF EILELVGFFK 

       250        260        270        280        290        300 
AAANLEEFCG GSLNEDIGMP EKYMNLVFPR KLCRLGLSYM GPNEMPILFP FAAQIRKLDL 

       310        320        330        340        350        360 
LYALLETEDH CTLIQKCPNL EVLETRNVIG DRGLEVLAQY CKQLKRLRIE RGADEQGMED 

       370        380        390        400        410        420 
EEGLVSQRGL IALAQGCQEL EYMAVYVSDI TNESLESIGT YLKNLCDFRL VLLDREERIT 

       430        440        450        460        470        480 
DLPLDNGVRS LLIGCKKLRR FAFYLRQGGL TDLGLSYIGQ YSPNVRWMLL GYVGESDEGL 

       490        500        510        520        530        540 
MEFSRGCPNL QKLEMRGCCF SERAIAAAVT KLPSLRYLWV QGYRASMTGQ DLMQMARPYW 

       550        560        570        580        590 
NIELIPSRRV PEVNQQGEIR EMEHPAHILA YYSLAGQRTD CPTTVRVLKE PI 

O04197 in FASTA format

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