[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). STRAIN=ICR; TISSUE=Embryonic tail; DOI=10.1093/dnares/11.2.127; PubMed=15449545 [NCBI, ExPASy, EBI, Israel, Japan] Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.; "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."; DNA Res. 11:127-135(2004).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=C57BL/6J; The mouse genome sequencing consortium; Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). STRAIN=FVB/N; TISSUE=Liver, and Mammary tumor; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity).
CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD⁺ + H⁺ + 2 ferrocytochrome b5.
COFACTOR: FAD (By similarity).

ALTERNATIVE PRODUCTS: 4 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	B1AS42-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	B1AS42-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_033851.

Name	3
Isoform ID	B1AS42-3
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_033849, VSP_033850.

Name	4
Isoform ID	B1AS42-4
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_033848, VSP_033852, VSP_033853.

SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AK220154; BAD90340.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL607132; CAM46300.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL607132; CAM46301.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC004750; AAH04750.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC043687; AAH43687.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_780680.1; -.

UniGene

Mm.44608

3D structure databases

ModBase

B1AS42.

Organism-specific databases

MGI

MGI:1919657; 2810410C14Rik.

Ontologies

GO:0004128;	Molecular function: cytochrome-b5 reductase activity (inferred from electronic annotation from EC).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Pfam

PF00970; FAD_binding_6; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PROSITE

PS51384; FAD_FR; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000028621; Mus musculus. [Contig view]

GeneID

230582; -.

KEGG

mmu:230582; -.

Other

379943; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; FAD; Flavoprotein; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 316`	`316`	`Probable NADH-cytochrome b5 reductase FLJ00377 homolog.`	`PRO_0000337042`
`DOMAIN`	`76 178`	`103`	`FAD-binding FR-type.`
`NP_BIND`	`158 173`	`16`	`FAD (By similarity).`
`NP_BIND`	`183 215`	`33`	`FAD (By similarity).`
`VAR_SEQ`	`1 85`		`Missing (in isoform 4).`	`VSP_033848`
`VAR_SEQ`	`1 60`		`Missing (in isoform 3).`	`VSP_033849`
`VAR_SEQ`	`61 67`		`KQPPESQ -> MNNKDLE (in isoform 3).`	`VSP_033850`
`VAR_SEQ`	`146 180`		`Missing (in isoform 2).`	`VSP_033851`
`VAR_SEQ`	`250 283`		`EVSPEQLPWSYRDKTHFGRLGQELVAELVACCRR -> ASPSPSYRGGSRLTEVLDQGGARTGPVCLTLLSL (in isoform 4).`	`VSP_033852`
`VAR_SEQ`	`284 316`		`Missing (in isoform 4).`	`VSP_033853`

Sequence information

Length: 316 AA [This is the length of the unprocessed precursor]

Molecular weight: 35884 Da [This is the MW of the unprocessed precursor]

CRC64: A5D49CF710DD7AFA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAETEEEEDS EAWLRLKPVE PLPSQCCGSG CSPCVFDLYY RDLERWETAR ARNDRSLLSG 

        70         80         90        100        110        120 
KQPPESQSCS AKLSPETFLA FHISTMEKVT KDTYLVRFTL PGNSRLGLRP GQHLILRGVV 

       130        140        150        160        170        180 
DGLEIQRAYT PISPVTAEGY FDVLIKCYRT GLMSQYVESW RTGDTAFWRG PFGSFLYEPK 

       190        200        210        220        230        240 
KYGELLMLAA GTGLAPMVPI LQSITDDEDD ETFVTLVGCF KTFEGIYLKT FFQEQARFWN 

       250        260        270        280        290        300 
VQTFFVLSQE VSPEQLPWSY RDKTHFGRLG QELVAELVAC CRRKPFTLVC GSPAFNEDMA 

       310 
RCLLSAGLTE DSYFLF

B1AS42 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools