NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nbt1325; PubMed=17704766 [NCBI, ExPASy, EBI, Israel, Japan]
Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R.;
"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42.";
Nat. Biotechnol. 25:1007-1014(2007).

Comments

CATALYTIC ACTIVITY: (R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.
CATALYTIC ACTIVITY: (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4 .
PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4 .
SIMILARITY: Belongs to the ketol-acid reductoisomerase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000560; ABS74895.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001422126.1; -.

3D structure databases

ModBase

A7Z7B9.

Ontologies

GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004455;	Molecular function: ketol-acid reductoisomerase activity (inferred from electronic annotation from HAMAP).
GO:0009097;	Biological process: isoleucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0009099;	Biological process: valine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00435; -; 1.
PBIL [Tree]

InterPro

IPR013023; AcH_isomrdctse.
IPR000506; AcH_isomrdctse_C.
IPR013116; IlvN.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR21371; AcH_isomrdctse; 1.

Pfam

PF01450; IlvC; 1.
PF07991; IlvN; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00465; ilvC; 1.

Genome annotation databases

GeneID

5462136; -.

GenomeReviews

CP000560_GR; RBAM_025350.

KEGG

bay:RBAM_025350; -.

CMR

A7Z7B9; RBAM_025350.

Other

ProtoNet

A7Z7B9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 342`	`342`	`Ketol-acid reductoisomerase.`	`PRO_1000050479`
`ACT_SITE`	`107 107`		`Potential.`

Sequence information

Length: 342 AA [This is the length of the unprocessed precursor]

Molecular weight: 37473 Da [This is the MW of the unprocessed precursor]

CRC64: 360EE4AFB8ECE588 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVKVYYNGDI KENVLAGKKV AIIGYGSQGH AHALNLKESG IDVIVGVRQG KSFTQAQEDG 

        70         80         90        100        110        120 
HQVFSVREAA AQADIIMVLL PDEQQQKVYE AEIKDELTAG KSLVFAHGFN VHFHQIVPPA 

       130        140        150        160        170        180 
DVDVFLVAPK GPGHLVRRTY EQGAGVPALF AIYQDVSGEA KDTALAYAKG IGGARAGVLE 

       190        200        210        220        230        240 
TTFKEETETD LFGEQAVLCG GLTALVKAGF ETLTEAGYQP ELAYFECLHE LKLIVDLMYE 

       250        260        270        280        290        300 
EGLAGMRYSI SDTAQWGDFV SGPRVVDAKV KESMKQVLTD IQNGTFAKEW IVENQVNRPR 

       310        320        330        340 
FNAINASENE HQIEKVGRQL REMMPFVKQG KKKEAVVSVA QN

A7Z7B9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools