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| PDOC00073 |
Pyridine nucleotide-disulphide oxidoreductases class-I active site
Description:
The pyridine nucleotide-disulphide oxidoreductases are FAD flavoproteins which
contains a pair of redox-active cysteines involved in the transfer of reducing
equivalents from the FAD cofactor to the substrate. On the basis of sequence
and structural similarities [1] these enzymes can be classified into two
categories. The first category groups together the following enzymes [2,3,4,5,6]:
- Glutathione reductase (EC 1.8.1.7) (GR).
- Higher eukaryotes thioredoxin reductase (EC 1.8.1.9).
- Trypanothione reductase (EC 1.8.1.12).
- Lipoamide dehydrogenase (EC 1.8.1.4), the E3 component of α-ketoacid
dehydrogenase complexes.
- Mercuric reductase (EC 1.16.1.1).
The sequence around the two cysteines involved in the redox-active disulfide
bond is conserved and can be used as a signature pattern.
Note:
In positions 6 and 7 of the pattern all known sequences have Asn-(Val/
Ile) with the exception of GR from plant chloroplasts and from cyanobacteria
which have Ile-Arg [7].
Last update:
May 2004 / Text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| PYRIDINE_REDOX_1, PS00076; Pyridine nucleotide-disulphide oxidoreductases class-I active site (PATTERN) |
| Consensus pattern: |
G - G - x - C - [LIVA] - x(2) - G - C - [LIVM] - P
The 2 C's form the active site disulfide bond |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1AOG 1BWC 1BZL 1DXL ... [ALL] |
References:
| 1 |
Authors | Kurlyan J., Krishna T.S.R., Wong L., Guenther B., Pahler A., Williams C.H. Jr., Model P. |
| Source | Nature 352:172-174(1991). |
| 2 |
Authors | Rice D.W., Schulz G.E., Guest J.R. |
| Title | Structural relationship between glutathione reductase and lipoamide dehydrogenase. |
| Source | J. Mol. Biol. 174:483-496(1984). |
| PubMed ID | 6546954 |
| 3 |
Authors | Brown N.L. |
| Source | Trends Biochem. Sci. 10:400-402(1985). |
| 4 |
Authors | Carothers D.J., Pons G., Patel M.S. |
| Title | Dihydrolipoamide dehydrogenase: functional similarities and divergent evolution of the pyridine nucleotide-disulfide oxidoreductases. |
| Source | Arch. Biochem. Biophys. 268:409-425(1989). |
| PubMed ID | 2643922 |
| 5 |
Authors | Walsh C.T., Bradley M., Nadeau K. |
| Title | Molecular studies on trypanothione reductase, a target for antiparasitic drugs. |
| Source | Trends Biochem. Sci. 16:305-309(1991). |
| PubMed ID | 1957352 |
| 6 |
Authors | Gasdaska P.Y., Gasdaska J.R., Cochran S., Powis G. |
| Title | Cloning and sequencing of a human thioredoxin reductase. |
| Source | FEBS Lett. 373:5-9(1995). |
| PubMed ID | 7589432 |
| 7 |
Authors | Creissen G., Edwards E.A., Enard C., Wellburn A., Mullineaux P. |
| Title | Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.). |
| Source | Plant J. 2:129-131(1992). |
| PubMed ID | 1303792 |
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